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Composite

Part:BBa_K781001:Design

Designed by: Kevin Chen   Group: iGEM12_Queens_Canada   (2012-10-02)


[R0010][B0034] - RFP-FliC Insertion Chimera


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2173
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 530
    Illegal AgeI site found at 938
    Illegal AgeI site found at 1552
    Illegal AgeI site found at 1664
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The linker and D3 overlap sequences are standard for our parts. By digesting the plasmid with the two inner enzymes, SpeI and NheI for our BB-2 standard parts, you can purify and then PCR overlap extend using the following protocol to result in a brand new chimera.


Source

This was created using overlap extension PCR to insert into K781000 at the annotated site. The insert sequence was amplified using PCR from J04450.

References